Evaluation of a Polymerase Chain Reaction-Based Assay for Diagnosis of Wuchereria bancrofti Infection
Identifieur interne : 00BE70 ( Main/Exploration ); précédent : 00BE69; suivant : 00BE71Evaluation of a Polymerase Chain Reaction-Based Assay for Diagnosis of Wuchereria bancrofti Infection
Auteurs : James S. Mccarthy [États-Unis] ; Min Zhong [États-Unis] ; Ramya Gopinath [États-Unis] ; Eric A. Ottesen [États-Unis] ; Steven A. Williams [États-Unis] ; Thomas B. Nutman [États-Unis]Source :
- Journal of Infectious Diseases [ 0022-1899 ] ; 1996-06.
Abstract
To assess the utility of a polymerase chain reaction (PCR)-based method for diagnosis of Wuchereria bancrofti infection, blood, plasma, and paraffin-embedded tissue samples were tested using a PCR-based assay that detects a W. bancrofti-specific repetitive DNA sequence. The assay was positive in 100 µL of blood from 40 of 42 microfilaria-positive subjects, the 2 subjects with negative assays having microfilarial counts of 1. Samples from 127 uninfected subjects were PCR-negative. The assay was also positive in 7 of 10 daytime samples in regions where infection is nocturnally periodic; PCR amplification from paraffin-embedded sections established the diagnosis of W. bancrofti infection in another 2 cases. A microtiter ELISA plate-based method was developed for rapid evaluation of large numbers of samples. These results suggest that this PCR-based assay will be useful in diagnosis of W. bancrofti infection in a variety of clinical settings.
Url:
DOI: 10.1093/infdis/173.6.1510
Affiliations:
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The assay was positive in 100 µL of blood from 40 of 42 microfilaria-positive subjects, the 2 subjects with negative assays having microfilarial counts of 1. Samples from 127 uninfected subjects were PCR-negative. The assay was also positive in 7 of 10 daytime samples in regions where infection is nocturnally periodic; PCR amplification from paraffin-embedded sections established the diagnosis of W. bancrofti infection in another 2 cases. A microtiter ELISA plate-based method was developed for rapid evaluation of large numbers of samples. These results suggest that this PCR-based assay will be useful in diagnosis of W. bancrofti infection in a variety of clinical settings.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Mccarthy, James S" sort="Mccarthy, James S" uniqKey="Mccarthy J" first="James S." last="Mccarthy">James S. Mccarthy</name></region><name sortKey="Gopinath, Ramya" sort="Gopinath, Ramya" uniqKey="Gopinath R" first="Ramya" last="Gopinath">Ramya Gopinath</name><name sortKey="Nutman, Thomas B" sort="Nutman, Thomas B" uniqKey="Nutman T" first="Thomas B." last="Nutman">Thomas B. Nutman</name><name sortKey="Ottesen, Eric A" sort="Ottesen, Eric A" uniqKey="Ottesen E" first="Eric A." last="Ottesen">Eric A. Ottesen</name><name sortKey="Williams, Steven A" sort="Williams, Steven A" uniqKey="Williams S" first="Steven A." last="Williams">Steven A. Williams</name><name sortKey="Zhong, Min" sort="Zhong, Min" uniqKey="Zhong M" first="Min" last="Zhong">Min Zhong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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